abstract：:A considerable number of animal studies on the effects of dopaminergic agents on alcohol intake behavior have been performed. Acute alcohol administration in rats induces dopamine release in the caudate nucleus and in the nucleus accumbens, an effect related among others to reinforcement. It has been repeatedly sugges...

journal_title：Journal of biomedical science

authors： Walter H,Ramskogler K,Semler B,Lesch OM,Platz W

更新日期：2001-01-01 00:00:00

abstract：:The envelope gene of Moloney murine leukemia virus (Mo-MLV) and its various functional domains have been studied extensively but not as much in terms of their biological effects on cell growth. In this study, we report the biological characterization of a truncated Mo-MLV envelope gene, LN11, which is devoid of a sign...

journal_title：Journal of biomedical science

authors： Chen H,Chung SW,Wong PM

更新日期：2000-11-01 00:00:00

abstract：:Based on the tertiary structure of the ribosome-inactivating protein alpha-sarcin, domains that are responsible for hydrolyzing ribosomes and naked RNA have been dissected. In this study, we found that the head-to-tail interaction between the first amino beta-strand and the last carboxyl beta-strand is not involved in...

journal_title：Journal of biomedical science

authors： Hwu L,Huang K,Chen D,Lin A

更新日期：2000-09-01 00:00:00

abstract：:Adeno-associated virus (AAV) vectors were shown capable of high efficiency transduction of both dividing and nondividing cells and tissues. AAV-mediated transduction leads to stable, long-term transgene expression in the absence of apparent immune response. These properties and the broad host range of AAV vectors indi...

journal_title：Journal of biomedical science

authors： Tal J

更新日期：2000-07-01 00:00:00

abstract：:The midbrain periaqueductal gray matter (PAG) is an important brain region for the coordination of mu-opioid-induced pharmacological actions. The present study was designed to determine whether newly isolated mu-opioid peptide endomorphins can activate G proteins through mu-opioid receptors in the PAG by monitoring th...

journal_title：Journal of biomedical science

authors： Narita M,Mizoguchi H,Narita M,Dun NJ,Hwang BH,Endoh T,Suzuki T,Nagase H,Suzuki T,Tseng LF

更新日期：2000-05-01 00:00:00

abstract：:To identify novel genes associated with apoptosis in glioma cells, we treated T98G glioma cells with okadaic acid (OA). Differential display using 15 random primers was performed on RNA extracted from these cells. Upregulated bands were excised from polyacrylamide gels and cloned. Northern blots were used to confirm R...

journal_title：Journal of biomedical science

authors： Chin LS,Singh SK,Wang Q,Murray SF

更新日期：2000-03-01 00:00:00

abstract：:Induction of tumoricidal activity is one of the major functions of activated macrophages. Our previous study demonstrated that P388D1 murine macrophage-like cells secreted a plasmacytoma cytotoxic factor (PCF) that selectively killed certain tumor cell lines including MOPC-315 plasmacytoma in vitro. Our subsequent stu...

journal_title：Journal of biomedical science

authors： Chu CY,Tseng J

更新日期：2000-01-01 00:00:00

abstract：:The HIV pol sequentially encodes protease (PR), reverse transcriptase (RT), and integrase (IN) from the 5'-3' direction. We explored the significance of this gene arrangement. All six possible gene dispositions were examined. In two situations where PR was removed from the leading place and no two genes were in their ...

journal_title：Journal of biomedical science

authors： Chang YY,Yu SL,Syu WJ

更新日期：1999-09-01 00:00:00

abstract：:The isoprostanes (IsoPs) are a unique series of prostaglandin-like compounds formed in vivo from the free radical-catalyzed peroxidation of arachidonic acid. This review summarizes our current knowledge regarding these compounds. Novel aspects of the biochemistry and bioactivity of IsoPs are detailed and methods by wh...

journal_title：Journal of biomedical science

authors： Liu T,Stern A,Roberts LJ,Morrow JD

更新日期：1999-07-01 00:00:00

abstract：:The dual signal hypothesis of apoptosis holds that a common signal can activate both apoptotic and proliferative pathways. The fate of a cell is dependent on which of these two pathways predominates. In the MAPK family of kinases, ERK and JNK have been proposed to mediate apoptosis whereas the PI3K-stimulated kinase, ...

journal_title：Journal of biomedical science

authors： Chin LS,Murray SF,Harter DH,Doherty PF,Singh SK

更新日期：1999-05-01 00:00:00

abstract：:Phospholipase D (PLD) is a phosphodiesterase that catalyses hydrolysis of phosphatidylcholine to produce phosphatidic acid and choline. In the presence of ethanol, PLD also catalyses the formation of phosphatidylethanol, which is a unique characteristic of this enzyme. Muscarinic receptor-induced changes in the activi...

journal_title：Journal of biomedical science

authors： Mamoon AM,Smith J,Baker RC,Farley JM

更新日期：1999-03-01 00:00:00

abstract：:Endotoxin shock is characterized by systemic hypotension, hyporeactiveness to vasoconstrictors and acute lung edema. A nitric oxide synthase (NOS) inhibitor, NG-monomethyl-L-arginine (L-NMMA) has been shown to be effective in reversing acute lung injury. In the present study, we evaluated the effects of NOS blockade b...

journal_title：Journal of biomedical science

authors： Wang D,Wei J,Hsu K,Jau J,Lieu MW,Chao TJ,Chen HI

更新日期：1999-01-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) latency-associated transcript (LAT) promoter 1 (LP1) is an inducible and cell type-specific promoter involved in regulating the production of an 8.3-kb primary LAT transcript during acute and latent infection of peripheral sensory neurons and during subsequent virus reactivation...

journal_title：Journal of biomedical science

authors： Millhouse S,Kenny JJ,Quinn PG,Lee V,Wigdahl B

更新日期：1998-11-01 00:00:00

abstract：:Previously we have reported the presence of endogenous ligands that are involved in the regulation of the binding of muscimol to the GABA binding site of the GABAA receptors. Here, we report the presence of multiple forms of endogenous ligands in the brain which modulate the binding of flunitrazepam (FNZP) to the benz...

journal_title：Journal of biomedical science

authors： Yarom M,Tang XW,Wu E,Carlson RG,Vander Velde D,Lee X,Wu J

更新日期：1998-07-01 00:00:00

abstract：:Protein kinases play pivotal roles in the development of the nervous system. They are involved in almost every stage of neuronal development, from initial proliferation and differentiation of progenitor cells to pathfinding of neurites and formation of synapses. Activation of protein kinases is also critical for neuro...

journal_title：Journal of biomedical science

authors： Lin SD,Fann MJ

更新日期：1998-01-01 00:00:00

abstract：:The HIV-1 Tat protein is an RNA-binding transcriptional transactivator. Recent findings suggest that Tat associates with a cellular kinase that phosphorylates the carboxyl-terminal domain of the largest subunit of RNA polymerase II. Here we review, in brief, the role of Tat-associated kinase in Tat-activated transcrip...

journal_title：Journal of biomedical science

authors： Jeang KT

更新日期：1998-01-01 00:00:00

abstract：:We have generated peptide antisera against selected regions in HIV-1 and HIV-2 reverse transcriptase (RT) and integrase (IN) to investigate the specificity of determinants governing the immune response. Peptides representing homologous regions (>50%) in the N- and C-termini and central portions of these proteins were ...

journal_title：Journal of biomedical science

authors： Klutch M,Woerner AM,Marcus-Sekura CJ,Levin JG

更新日期：1998-01-01 00:00:00

abstract：:Mitochondria are the major intracellular source and target sites of reactive oxygen species (ROS) that are continually generated as by-products of aerobic metabolism in animal and human cells. It has been demonstrated that mitochondrial respiratory function declines with age in various human tissues and that a defecti...

journal_title：Journal of biomedical science

authors： Lee HC,Wei YH

更新日期：1997-11-01 00:00:00

abstract：:Agents which block T cell K(+) currents can prohibit both proliferative and effector cell functions in T cells activated by mitogens or phorbol esters. This study examined the effects of some of these blocking agents on the immune responsiveness of guinea pig myelin basic protein (GPMBP)-reactive Lewis rat T lymphocyt...

journal_title：Journal of biomedical science

authors： Judge SI,Yeh JZ,Mannie MD,Pope Seifert L,Paterson PY

更新日期：1997-07-01 00:00:00

abstract：:Experimental allergic encephalomyelitis (EAE) is an accepted animal model for the human demyelinating disease multiple sclerosis. The continuously propagated line of Lewis rat T helper lymphocytes (GP1 T cells), specific for the encephalitogenic 68-86 sequence of guinea pig myelin basic protein (GPMBP), mediates the a...

journal_title：Journal of biomedical science

authors： Judge SI,Paterson PY,Mannie MD,Yeh JZ

更新日期：1997-03-01 00:00:00

abstract：:Sensory innervation of the skin subserves protective sensations for the body to prevent thermal and noxious injuries. Neurophysiologically, they belong to the categories of Adelta and C fibers, usually with caliber less than one micro m in diameter. Morphological demonstration of the terminals of these nerves in the e...

journal_title：Journal of biomedical science

authors： Hsieh ST,Lin WM,Chiang HY,Huang IT,Ko MH,Chang YC,Chen WP

更新日期：1997-01-01 00:00:00

abstract：:The Tat proteins of human immunodeficiency virus types 1 (HIV-1) and 2 (HIV-2), termed Tat-1 and Tat-2, respectively, are essential for efficient viral replication. Tat proteins activate viral transcription by binding to the TAR RNA stem-loop structure at the 5' end of viral transcripts. We used an in vitro selection ...

journal_title：Journal of biomedical science

authors： Rhim H,Rice AP

更新日期：1997-01-01 00:00:00

abstract：:Exposure of cells and organs to heat shock is associated with numerous changes in various cellular metabolic parameters and overexpression of proteins collectively known as heat shock proteins (HSP). In this communication we review the cell-signaling events that are altered in response to heat shock as they relate to ...

journal_title：Journal of biomedical science

authors： Gong Kiang J,Tsokos GC

更新日期：1996-11-01 00:00:00

abstract：:The purpose of this study was to determine whether beta-adrenergically mediated cardiovascular functions such as arterial pressure (AP), heart rate (HR), stroke volume (SV), cardiac output (CO), peripheral resistance (R(p)), arterial impedance (Z(c)), mean arterial compliance (C(m)) and pulse wave reflection (P(b)) we...

journal_title：Journal of biomedical science

authors： Hu CT,Wu CY,Chen HI

更新日期：1996-07-01 00:00:00

abstract：:The interactive role of rostral ventrolateral medulla (RVL) cardiovascular neurons and brain angiotensin II (Ang II) in regulating the arterial blood pressure was examined by recording simultaneously the spontaneous activity of these spinal projecting neurons and the arterial blood pressure in the pentobarbital-anesth...

journal_title：Journal of biomedical science

authors： Chan RK,Chan YS,Wong TM

更新日期：1996-05-01 00:00:00

abstract：:A three-dimensional molecular model of the transmembrane domain of the 5-HT(1A) receptor (5-HT(1A)R) is presented in the context of a general strategy for modeling the macromolecular structure of a guanine nucleotide binding, regulatory protein coupled receptor (GPCR). The model of the 5-HT(1A)R rests on the definitio...

journal_title：Journal of biomedical science

authors： Pardo L,Batlle M,Duñach M,Weinstein H

更新日期：1996-04-01 00:00:00

abstract：:Sequencing of HIV-1(GER) long terminal repeat (LTR) has demonstrated, for the first time in an HIV-1 primary isolate, a TAR duplication referred to as TAR1 (nucleotides +1 through +68) and TAR2 (nucleotides +69 through +136). This TAR duplication is stable during replication of HIV-1(GER) isolate in CEM cells. Analysi...

journal_title：Journal of biomedical science

authors： Emiliani S,Coudronnière N,Delsert C,Devaux C

更新日期：1996-01-01 00:00:00

abstract：:Our objective was to determine the role that bone marrow-derived stromal cells have on human hematopoiesis in HIV infection. In particular, we dissected the heterogeneous bone marrow microenvironment to study the effect HIV expression might have on the cell population capable of producing the cytokines which will supp...

journal_title：Journal of biomedical science

authors： Mosca JD,Kaushal S,Gartner S,Kessler SW,La Russa VF,Terwilliger EF,Kim JH,Carroll RG,Hall ER,Perera LP,Yu Z,Ritchey DW,Xu J,St Louis DC,Mayers DL

更新日期：1995-10-01 00:00:00

abstract：:The aim of this study was to determine whether the common forms of dyslipidemia could affect either the lipid composition or insulin receptor processing (down-regulation) of erythrocytes. The study included 22 patients with type IIa hypercholesterolemia, 15 patients with type IV hypertriglyceridemia and 12 patients wi...

journal_title：Journal of biomedical science

authors： Masella R,Cantafora A,Maffì D,Volpe R,Ginnetti MG,Ricci G,Mace NL,Buxton GM,Peterson SW

更新日期：1995-08-01 00:00:00

abstract：:The likelihood that expression of a foreign gene in a mammalian cell is deleterious to viability is confronted whenever novel transgenic animals are made. A pathological response to transgene expression is even desired in transgenic mouse models of human disease. The derivation of HIV-transgenic mice in our laboratory...

journal_title：Journal of biomedical science

authors： Dickie P

更新日期：1995-04-01 00:00:00

abstract：:The apolipoprotein (Apo) E gene is known to be polymorphic. Three common alleles determine six phenotypes which can easily be detected by restriction fragment length polymorphism. We performed apo E genotyping in myocardial infarction survivors and healthy controls for the first time in the Turkish population. DNA was...

journal_title：Journal of biomedical science

authors： Hergenc G,Taga Y,Emerk K,Cirakoglu B

更新日期：1995-01-01 00:00:00

abstract：:Two Chinese hamster ovary (CHO) cell lines stably transfected with human insulin receptor cDNA, CHO-wt and CHO-mut, which express an equivalent number of normal and kinase-defective human insulin receptors, respectively, were used to assess the roles of insulin receptor tyrosine kinase activity in insulin-regulated ge...

journal_title：Journal of biomedical science

authors： Lin SC,Chen MF,Chou CK

更新日期：1994-12-01 00:00:00

abstract：:Glucocorticoids regulate the expression of many liver-specific genes via glucocorticoid receptors. The presence of glucocorticoid receptors in liver has been reported in many mammalian species but not in nude mice. In the present study, we demonstrate the presence of specific glucocorticoid receptors in nude mouse liv...

journal_title：Journal of biomedical science

authors： Chi CW,Liu TY,Chiang SH,Cheng SL,Lin CY,Ho CK

更新日期：1994-10-01 00:00:00

abstract：:The nucleotide sequences of the putative envelope region (E1) and the junction between the E1 and envelope 2/nonstructural 1 (E2/NS1) region of the hepatitis C virus (HCV) genome are divergent among different genotypes. To characterize them, we introduced a set of nested primers that are conserved among four different...

journal_title：Journal of biomedical science

authors： Kao JH,Chen PJ,Lai MY,Yang PM,Sheu JC,Wang TH,Chen DS

更新日期：1994-06-01 00:00:00

abstract：:The ACTGCTGA sequence (CTG motif) is located immediately upstream of the NF-kappaB enhancer in the human immunodeficiency virus type-1 (HIV-1) long terminal repeat (LTR). We previously reported on the frequent duplication of this motif in HIV-1-infected individuals. In this study we further characterized the role of t...

journal_title：Journal of biomedical science

authors： Koken SE,van Wamel JL,Geelen JL,Berkhout B

更新日期：1994-03-01 00:00:00